FIG. 2.

Confocal immunofluorescence analysis of the intracellular distribution of HCV glycoproteins. Infected cells grown on coverslips were fixed and processed for double-label immunofluorescence for E1, E2, and the following cellular markers: calnexin, a chaperone of the ER; GM130, a Golgi matrix protein; ERGIC-53, a marker of the ER-to-Golgi intermediate compartment; or LAMP-1, a marker of late endosomes and lysosomes. Anti-E2 mouse MAb AP33 was used for the colocalization with E1. For the other experiments, E2 was revealed with the rat MAb 3/11. Representative confocal images of individual cells are shown with the merge images in the right column. Bar, 20 μm.