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Conversion of Feruloyl-CoA to Vanillin by the HCHL Enzyme.
HCHL performs the hydration and cleavage of feruloyl-CoA to produce vanillin and acetyl-CoA in vitro. The enzyme also is able to reproduce feruloyl-CoA, in addition to vanillin and acetyl-CoA, from the artificial substrate and putative reaction intermediate 4-hydroxy-3-methoxyphenyl-β-hydroxypropionyl-CoA (HMPHP-CoA) (Gasson et al., 1998).
