Steady State mRNA Levels of the HCHL Transcript in Independent Primary Transformants.
Total RNA from young leaves of primary transformants was hybridized to the HCHL gene fragment. Lanes 1 to 12, plants containing the pmHCHL construct: plants 3, 6, 7, 9, 11, 13, 21, 22, 27, 34, 37, and 39; lane 13, no sample; lanes 14 to 16, plants containing the pHCHL construct: plants 201, 210, and 224; lanes 17 and 18, control plants transformed with the empty pBin19 vector. The same blot was stripped and hybridized to a Datura stramonium ubiquitin probe.