Figure 3.

Morphology and Microscopic Analysis of Transgenic Plants.

(A) Primary transformant 201 at 22 days after transfer to the greenhouse.

(B) Detached leaf of primary transformant 201 at 74 days after transfer (after flowering).

(C) to (I) Comparisons between transgenic and syngenic progeny of line 201. (C) shows sibling plants of line 201 at 90 days after sowing, demonstrating delayed elongation of stem internodes in transgenic plants (left). Unstained stem sections showing an orange/red coloration of the vascular ring in transgenic plants (D) compared with the yellow color observed in syngenic plants (G) and distortion of phloem fibers in the transgenic stem (E) compared with the syngenic plants (H) were viewed under UV light fluorescence. Phloroglucinol staining of vascular tissue in transgenic stems (F) was reduced compared with that of the syngenic samples (I).