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. 2001 Jul;13(7):1669–1682. doi: 10.1105/TPC.010063

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Copyright © 2001, American Society of Plant Biologists

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Figure 5. — HCHL Enzyme Synthesis in Young Leaves of Transgenic Progeny and Activity in Vitro.

(A) Protein gel blot analysis of total protein extracts from lines 201 and 27. Lane 1, transgenic, line 201, set 1; lane 2, syngenic, line 201, set 1; lane 3, transgenic, line 201, set 2; lane 4, syngenic, line 201, set 2; lane 5, transgenic, line 27; lane 6, syngenic, line 27; lane 7, total protein extract from Pseudomonas strain AN103. Line 201 was sown on two separate occasions (sets 1 and 2).

(B) In vitro production of vanillin (V) by the incubation of 10 μL of cell-free extracts from a transgenic offspring (T) of line 201 with feruloyl-CoA (F) at 30°C for 0, 3, 5, and 10 min. Incubation with extracts from a syngenic plant (N) showed no accumulation of vanillin at any time. HPLC analysis was viewed at 310 nm.