FIG. 3.

RV infection prevents IFN-mediated translocation of STAT1 (A) and STAT2 (B) to the nucleus. RV-infected (MOI of 1) and mock-infected Hep-2 cells were stimulated with IFN-γ or IFN-α for 45 min at 24 h postinfection, fixed with 3% paraformaldehyde, and stained with STAT1 or STAT2 antibodies as indicated. The import of both STATs on IFN-treated cells is prevented in RV-infected cells, whereas import of NF-κB p65 on TNF-α-treated cells is not (C). Nuclei of cells were visualized by staining with TO-PRO-3 dye; RV P was visualized by using P-specific polyclonal mouse serum.