. Author manuscript; available in PMC: 2006 Mar 9.

Published in final edited form as: J Biol Chem. 2005 Sep 29;280(47):39474–39484. doi: 10.1074/jbc.M504672200

TABLE THREE.

Effects of external stimulation on platelet surface area following adhesion to collagen and laminin Purified human and murine platelets (2 × 107/ml) were placed on coverslips coated with collagen or laminin for 45 minutes. Platelets were treated with apyrase (2 units/ml) and indomethacin (10 μM) in the absence or presence of 1 unit/ml thrombin. In separate experiments, platelets were treated with 10 μM ADP in the absence of inhibitors of secondary mediators. Values are reported as follows: platelet surface area = mean ± S.E. of at least 250 cells.

Murine platelet spreading on collagen (SA; μm²)
Treatment	WT	Rac1^-/-Rac2^-/-
	18.1 ± 0.18	7.8 ± 0.07^a
ADP	18.4 ± 0.09	7.5 ± 0.18^a
Thrombin	18.3 ± 0.08	12.1 ± 0.12^a
Murine platelet spreading on laminin (SA; μm²)
Treatment	WT	Rac1^-/-Rac2^-/-
	23.9 ± 0.27	7.4 ± 0.06^a
ADP	22.9 ± 0.26	7.0 ± 0.09^a
Thrombin	23.5 ± 0.18	17.3 ± 0.10^a

p < 0.01 with respect to no treatment control for wild-type platelets.