TABLE 3.
Transformation of the disruptant strains with plasmids carrying multiple XhoI sites
| Expt | Recipient straina | No. of Cmr transformants (102)/μg of DNA with:
|
No. of transformants with pHV1401/ no. of transformants with pHV33 | Ratio | |
|---|---|---|---|---|---|
| pHV33 | pHV1401 | ||||
| 1 | 168 | 226 | 24 | 0.11 | 1.0 |
| BSU1 (ydiO ydiS) | 75 | 324 | 4.32 | 40.7 | |
| BSU2 (ydiP ydiS) | 12 | 110 | 9.17 | 86.3 | |
| BSU3 (ydiR) | 38 | 128 | 3.37 | 31.7 | |
| BSU4 (ydiS) | 20 | 46 | 2.30 | 21.7 | |
| BSU5 (ydjA) | 13 | 54 | 4.17 | 39.1 | |
| 2 | 168 | 134 | 2 | 0.01 | 1.0 |
| BSU9 [Δter (groESL)] | 135 | 82 | 0.61 | 61.0 | |
| 3 | 168 | 1.00 | 0.063 | 0.063 | 1.0 |
| BSU1 (ydiO ydiS) | 4.75 | 4.93 | 1.04 | 16.5 | |
| BSU2 (ydiP ydiS) | 0.38 | 0.38 | 1.00 | 15.8 | |
| BSU3 (ydiR) | 3.50 | 3.87 | 1.11 | 17.6 | |
| BSU4 (ydiS) | 3.12 | 3.94 | 1.26 | 20.0 | |
| BSU5 (ydjA) | 8.75 | 9.69 | 1.11 | 17.6 | |
| BSU9 [Δter (groESL)] | 4.69 | 3.94 | 0.84 | 13.3 | |
a
Each strain was transformed to resistance to chloramphenicol (5 μg/ml) with plasmid pHV33 or pHV1401. The genotypes in parentheses or brackets indicate the disrupted genes.