Comparison of partial RubisCO large-subunit sequences from 14 strains of AOB (for accession numbers see Materials and Methods). (A) Deduced amino acid sequence alignment compared to the corresponding regions in the CbbL proteins of R. eutropha ATCC 17707 (accession number M17744) (6), Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans) (30) ATCC 23270 (AF129925) (J. M. Shively, unpublished data, 1999), and Nitrobacter vulgaris T3 (L22885) (M. Strecker, E. Sickinger, R. S. English, J. M. Shively, and E. Bock, unpublished data). The translated sequences were aligned by using ClustalW (44). Identical residues are indicated by asterisks, conservative substitutions are indicated by colons, and semiconservative substitutions are indicated by periods. The active site residue Asn126 (R. eutropha numbering) is indicated. (B) Percentages of identity for the CbbL amino acid sequences in panel A (upper right) and for the cbbL nucleotide sequences (lower left). Levels of identity were calculated by using the HOMOLOGIES program (Genetics Computer Group). Abbreviations: Nsp, Nitrosospira; Nc, Nitrosococcus; Nm, Nitrosomonas.