FIG. 3.

Construction and PGA productivity of gene disruptants. (A) Structures of ywsC, ywtA, and ywtB gene disruptants. The cat gene and spac promoter are indicated by a solid box and a solid arrow, respectively. The position of the putative hairpin structure is indicated by an open circle. (B) SDS-PAGE analysis of PGA production. Cells were grown in PGA medium, and the culture supernatant was subjected to SDS-PAGE. Lane 1, protein markers; lane 2, wild-type strain; lane 3, ΔywsC strain; lane 4, ΔywtA strain; lane 5, ΔywtB strain.