FIG. 4.

Production of YwsC-His in B. subtilis ΔywsC strain. (A) SDS-PAGE analysis of PGA production by B. subtilis IFO16449 and transformants. Cells were grown in PGA medium, and the culture supernatant was subjected to SDS-PAGE. (B) Western blot analysis of YwsC-His. Proteins prepared from the wild-type strain and transformants were separated by SDS-PAGE, and YwsC-His was detected with an Ni-NTA-AP conjugate. Arrows indicate the positions of subunits of YwsC-His. Lane 1, protein markers; lane 2, wild strain; lane 3, ΔywsC strain; lane 4, ΔywsC/pYWSC strain; lane 5, ΔywsC/pYWSC-Sac strain. The positions of the 33- and 44-kDa proteins are shown (arrows).