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SDS-PAGE of purified YwsC-His. Cells were grown in PGA medium (lanes 2 and 4) or Spizizen minimal medium containing 2% l-glutamate (lane 3). Purification of YwsC-His is described in Materials and Methods. Lane 1, protein markers; lanes 2 and 3, ΔywsC/pYWSC strain; lane 4, ΔywsC/pYWSC-Sac strain.
