FIG. 7.
Detection of the products of ATP cleavage. The polyglutamate synthetase reaction was carried out as described in Materials and Methods. At the times indicated, 10 μl of the reaction mixture was chromatographed on a polyethyleneimine-cellulose thin-layer plate and developed in sodium formate. Nucleotides were detected by their UV absorption, and radioactivities of labeled ATP (Rf 0.31), ADP (Rf 0.64), and AMP (Rf 0.83) were determined using a liquid scintillation counter. Symbols: •, ADP; ○, AMP.