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Phosphotyrosine peptide-based phosphatase assay. The amount (in picomoles) of free phosphate generated at 37°C by 45 μg of cellular protein (50 μl cell lysate) from Rx1-19F, Rx1-19F-BΔ, Rx1-19F-BNQ, and Rx1-19F-DΔ was measured at the indicated time points. The phosphopeptides pY-pep 1 (END[pY]INASL) and pY-pep 2 (DADE[pY]LIPQQG) were added at a final concentration of 100 μM to the lysates where indicated. Data are the means of three independent assays ± the SEM.
