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Dephosphorylation of CpsD-P by CpsB. Cell lysates (50 μl containing 45 μg of protein) from Rx1-19F-BΔ, Rx1-19F-DΔ, or both lysates combined were incubated at 37°C, and the amount of free phosphate (pmol) was determined at the indicated times. Symbols: □(, Rx1-19F-BΔ-Rx1-19F-DΔ lysate mix; ○, Rx1-19F-DΔ lysate; ▵, Rx1-19F-BΔ lysate. Data are the means of three independent assays ± the SEM.
