TABLE 4.
Activation function and trans-dominant effects of VirF proteins with mutations in the second HTH motif
| Plasmida | Mean (SD) of β-galactosidase activity (Miller units)b
|
|||
|---|---|---|---|---|
| CJD1006 at 30°C | CJD1006 at 37°C | BS184 at 37°C | BS184 + pMEP532 at 37°Cc | |
| pMEP510 | 700 (28.0) | 1,247 (50.0) | 1,574 (65.5) | 1,292 (51.5) |
| pV228T | 15 (0.50) | 30.8 (1.05) | 1,296 (67.0) | 1,234 (48.0) |
| pY239F | 10 (0.20) | 16.4 (0.10) | 1,318 (34.0) | 1,202 (48.0) |
| pI241N | 10 (0.20) | 16.9 (1.00) | 1,501 (42.0) | 1,467 (59.5) |
a
Transformed into the S. flexneri virF mutant strain CJD1006 or wild-type strain BS184. pMEP510 is the wild-type virF+ plasmid; mutations encoded by the other plasmids are indicated by single-letter amino acid codes.
b
β-Galactosidase activity data of the mxiC-lacZ fusion are mean results of duplicate assays on duplicate cultures.
c
Plasmid pMEP532 carries the wild-type virF gene.