TABLE 2.
Virulence of S. enterica recombination mutants in BALB/c mice
| Strain | Relevant genotype | LD50 (oral)a |
|---|---|---|
| SV1445 | Wild type | 1.0 × 105 |
| SV4191 | recA1 | <3.0 × 108 |
| SV4188 | recB497::MudJ | >7.2 × 109 |
| SV4212 | recD543::Tn10dCm | <7.6 × 106 |
| SV4213 | recF522::Tn5 | <7.4 × 106 |
| SV4192 | recJ504::MudJ | <2.0 × 106 |
| SV4496 | recA1 recF522::Tn5 | <6.5 × 108 |
| SV4497 | recA1 recJ504::MudJ | <3.5 × 108 |
| SV4498 | recB503::Tn10 recF522::Tn5 | >5.0 × 109 |
| SV4499 | recF522::Tn5 recJ504::MudCm | <8.7 × 105 |
| SV4363 | recD543::Tn10dCm recF522::Tn5 | <6.9 × 106 |
| SV4364 | recD543::Tn10dCm recJ504::MudJ | >2.6 × 108 |
a
Bacterial suspensions were prepared as described elsewhere (9). The acidic pH of the stomach was buffered by suspending bacteria in a 2.5% bicarbonate-0.2% lactose solution prior to administration. Survival of bacterium-infected mice was recorded for a minimum of 4 weeks. The LD50 was calculated by the method of Reed and Muench (30).