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HPLC analysis of naphthol productsa
| Enzyme | % Oxidized naphthalene
|
||
|---|---|---|---|
| 1-Naphthol | 2-Naphthol | Unknown | |
| Wild-type TOM | 98 | 0 | 2 |
| TOM-Green | 97 | 1 | 2 |
| Toluene-o-xylene monooxygenaseb | 74 | 25 | 1 |
| Toluene-3-monooxygenasec | 48 | 49 | 3 |
All plasmids were expressed in E. coli TG1.
From pBZ1260, encoding the touABCDEF gene cluster of Pseudomonas stutzeri OX1 (6).
From pRO1966, encoding tbuA1UBVA2C gene cluster of Pseudomonas pickettii PKO1 (4).
