TABLE 2.

Chlorinated ethene degradation and chloride produced by TG1 cells expressing wild-type TOM and the mutant TOM-Green^a

Compound	Initial liquid concn (μM)	Initial degradation rate (nmol/min/mg of protein)		Chloride produced (μM)
		Wild-type TOM	TOM- Green	Wild-type TOM	TOM- Green
TCE	67	1.39 ± 0.05	2.5 ± 0.3	131 ± 19	366 ± 48
1,1-DCE	25	0.42 ± 0.07	1.0 ± 0.2	61 ± 7	74 ± 1
cis-DCE	79	2.97	3.08	110 ± 6	107 ± 9
trans-DCE	86	1.13 ± 0.06	1.60 ± 0.2	56.9 ± 0.4	92 ± 5
VC	34	2.06	2.08	86.2 ± 0.6	99 ± 4
Toluene	22	0.64 ± 0.04	0.44 ± 0.08	N/Ab	N/A

^aChloride concentrations were determined by quenching reactions so that roughly 50% of each chlorinated ethene was oxidized by TOM-Green. Initial liquid concentrations indicated were calculated based on Henry’s law (2, 25) (200 μM added if all the volatile organic was in the liquid phase). The mean ± 1 standard deviation is shown.

^bN/A, not applicable.