FIG. 4.
Determination of transcriptional start sites of ORFs HP1408 and HP119 by primer extension analysis. Total RNA extracted from H. pylori G27/P1408-gfp (A) and G27 (B) were hybridized to radiolabeled oligonucleotides gfpPE and HP119PE, respectively. Transcriptional start points are indicated by arrows on the right. Parts of the respective promoter sequences are shown on the left. The −10 promoter elements are enclosed in boxes. Sequencing reactions (lanes A, C, G, and T) were performed by using the oligonucleotides used in the primer extension experiments and plasmid pSL-P1408-gfp/km (A) or pSL-119PE2 (B) as the template.