TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Reference or source |
|---|---|---|
| E. coli strains | ||
| CC118 | araD139 Δ(ara leu)7697 ΔlacX74 ΔphoA20 galE galK thi rpsE rpoB argE(Am) recA1 | 37 |
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR | 58 |
| SM10λpir | thi-1 thr leu tonA lacY supE recA::RP4-2-Tc::Mu (λpir) | 43 |
| AA60 | CC118, pTSS-5 | This study |
| AJA1 | CC118, pAJA1 | This study |
| AJA2 | CC118, pAJA2 | This study |
| AJA3 | CC118, pAJA3 | This study |
| RRH26 | CC118, pRH8 | This study |
| RRH72 | CC118, pRH72 | This study |
| RRH81 | CC118, pRH81 | This study |
| RRH82 | CC118, pRH82 | This study |
| RRH98 | CC118, pRH98 | This study |
| RRH100 | MC4100 (λtcpA-lacZYA) (−458/+2) | This study |
| RRH102 | MC4100 (λtcpA-lacZYA) (−162/+2), T to A at −38 | This study |
| RRH103 | MC4100 (λtcpA-lacZYA) (−162/+2), A to T at −50 | This study |
| RRH113 | MC4100 (λtcpA-lacZYA) (−95/+2) | This study |
| RRH114 | MC4100 (λtcpA-lacZYA) (−63/+72) | This study |
| RRH115 | MC4100 (λtcpA-lacZYA) (−162/+2) | This study |
| RRH131 | MC4100 (λtcpA-lacZYA) (−118/+2) | This study |
| V. cholerae strains | ||
| O395 Sm | Classical Ogawa, Smr | 63 |
| CG842 | O395 Sm ΔlacZ | 8 |
| MBN135 | CG842 tcpA-lacZ | M. Nye |
| MBN142 | MBN135 ΔtoxT | M. Nye |
| MBN168 | MBN135 ΔtoxT Δhns | M. Nye |
| RRH175 | MBN135 (pRH170) | This study |
| RRH176 | MBN135 (pRH171) | This study |
| RRH178 | MBN142 (pRH170) | This study |
| RRH179 | MBN142 (pRH171) | This study |
| RRH181 | MBN168 (pRH170) | This study |
| RRH182 | MBN168 (pRH171) | This study |
| MBN032 | CG842 toxT-lacZ | 49 |
| RRH197 | MBN032 (pRH170) | This study |
| RRH201 | MBN032 (pRH171) | This study |
| Plasmids | ||
| pTSS-5 | pACYC184 toxT+, Cmr | 3 |
| pRS415 | lacZYA transcriptional fusion vector, Apr | 59 |
| pAJA1 | pRS415::(tcpA-lacZYA) (−118/+2) | This study |
| pAJA2 | pRS415::(tcpA-lacZYA) (−162/+2) | This study |
| pAJA3 | pRS415::(tcpA-lacZYA) (−458/+2) | This study |
| pRH8 | pRS415::(tcpA-lacZYA) (−95/+2) | This study |
| pRH72 | pRS415::(tcpA-lacZYA) (−230/+2) | This study |
| pRH82 | pRS415::(tcpA-lacZYA) (−310/+2) | This study |
| pRH98 | pRS415::(tcpA-lacZYA) (−63/+72) | This study |
| pCJ1.3 | pRS415::(tcpA-lacZYA) (−162/+2), T to A at −38 | This study |
| pCJ10.4 | pRS415::(tcpA-lacZYA) (−162/+2), A to T at −50 | This study |
| pBAD22 | Expression plasmid, Apr | 21 |
| pRH81 | pBAD22 six-His toxT | This study |
| pMMB66EH | Expression plasmid, Apr | 17 |
| pRH170 | Full-length rpoA in pMMB66EH | This study |
| pRH171 | rpoAΔ235 in pMMB66EH | This study |