FIG. 2.
Stability of dinitrogenase reductase during oxygen treatment. Extracts were analyzed by SDS-PAGE and Western blotting using antibodies against dinitrogenase reductase from R. rubrum. (A) Extracts were prepared from cells (OD600 = 0.6) incubated under 20% oxygen in N2 with pyruvate as the carbon source for 2 (lane 1), 4 (lane 2), and 6 (lane 3) h. (B) Extracts were prepared from cells (OD600 = 0.6) incubated with 20% oxygen in N2 with pyruvate as the carbon source for 1 h, after which the atmosphere was changed to 5% oxygen in N2. Samples were taken after incubation for another 2 (lane 1), 4 (lane 2), and 6 (lane 3) h. The same amount of total protein was loaded in all six lanes.