SDS-PAGE profiles and zymogram of recombinant DSR-E produced by E. coli TOP10. Lanes: C, cellular extract, pBAD/TOPO Thiofusion, and Coomassie staining (negative control); 1, cellular extract, pBAD-dsrE, and Coomassie staining; 2, cellular extract, pBAD-dsrE, and periodic acid-Schiff staining (loading 5 mU of activity); M, broad-range prestained precision protein standard (Bio-Rad).