TABLE 1.
Oligoprimers used for PCR amplification
| Designation | Descriptiona | Sequence (5′-3′)b |
|---|---|---|
| 29-dir | FYFESGK | TT(C/T)TA(C/T)TT(C/T)GA(A/G)TCAGG(C/G)AA(A/G) |
| 24-inv | FESQNNNP | (T/G)GG(G/A)TT(G/A)TT(G/A)TTTTGTGA(T/C)TCAAA |
| IPCR-rev | nt 6334-6363 | CCCTTTACAAGCTGATTTTGCTTATCTGCG |
| IPCR-dir | nt 8876-8907 | GGGTCAAATCCTTACTATACATTGTCACACGG |
| pBAD-PS/ZV-dir | nt 677-706 | GCCATGGCAAATACGATTGCAGTTGACACG |
| pBAD-ent-rev | nt 8482-8505 | AATTTGAGGTAATGTTGATTTATC |
a
nt, nucleotides.
b
Primer sequences are designed from the noncoding strand (IPCR-dir or ECHO-dir) or from the complementary strand (IPCR-rev or ECHO-inv).