TABLE 2.
Allele | Position of mutation (bp) | Type of mutation | DNA change | Result of mutation |
---|---|---|---|---|
hisC952 | 450 | Transition | C→T | Asp→Aspb |
799 | Transversion | T→G | Tyr→Aspb | |
952 | Transition | C→T | Gln→stop (amber mutation) | |
metB5 | 346 | Transversion | G→T | Glu→stop (ochre mutation) |
leuC427 | 427 | Transition | G→A | Gly→Arg (missense mutation) |
Mutant alleles found in strain YB955 and the changes in the DNA sequences of the mutant alleles compared to the published genome of B. subtilis (http://genolist.pasteur.fr/SubtiList).
These point mutations in the hisC952 allele do not play any significant role in the inactivity of the gene product. These two changes may actually represent errors in the published genome.