TABLE 4.

DNA primers used for PCR and DNA sequencing reactions

Usea	Gene	5′ primer	3′ primer	DNA sequenceb	Used for PCRc	Used for sequencingd
Entire gene	hisC	+		5′ ATCAGGCGCTGCAGGAGTTTGAGG 3′	+	+
			+	5′ GACCGGCGAGCAATATTGTATCTTTCA 3′	+	+
Internal	hisC	+		5′ CAAACACGGTTACTGCTGC 3′		+
		+		5′ AAGGCATACGGTCTGGCAG 3′		+
Entire gene	metB	+		5′ AACTGCAGAAACGGGGAAATAATGGAGGTG 3′	+	+
			+	5′ CGGGATCCGGTGCCCTGTCAAAAAGACTTG 3′	+	+
Internal	metB	+		5′ TGCGCCGATCGAGCATTTGG 3′		+
			+	5′ CATGATTTCCTTGAGCTCTTCCCA 3′		+
Entire gene	leuC	+		5′ ACGCGTCGACAATACAATTTCTAATGTGTGACAG 3′	+	+
			+	5′ ACGCGTCGACATCCGATTTAATACGGGTGCTTTCC 3′	+	+
Internal	leuC	+		5′ CAGTGTGGATCAAGGGATTGTC 3′		+
		+		5′ ACGATGGATGAACGAATGACTG 3′		+
		+		5′ TTGAAGACATTAAAGTGGAGCAC 3′		+

^aEntire gene, primers used for sequencing of the entire genes used for stationary-phase mutagenesis assay; Internal, primers used for internal sequencing of the gene.

^bBold sequences are not part of the coding region of the gene but were used for cloning purposes.

^cPrimers used in PCRs to amplify the genes, as described in Materials and Methods.

^dPrimers used for sequencing as described in Materials and Methods.