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. 2002 Oct;184(20):5661–5671. doi: 10.1128/JB.184.20.5661-5671.2002

FIG. 2.

FIG. 2.

Analysis of htrB expression in a cssS mutant background. The CssS dependence of htrB-lacZ transcription was analyzed in the absence (A) or presence (B) of pKTH10L-induced secretion stress. The transcriptional htrB-lacZ gene fusion schematically shown in Fig. 1A was used to determine the time courses of htrB expression in cells grown at 37°C in TY medium. The strains used for the analyses were B. subtilis 168 htrB::pMutin4 (BFA3041; solid rectangles), B. subtilis 168 cssS::Sp htrB::pMutin4 (BV2015; open rectangles), B. subtilis 168 htrB::pMutin4 containing pKTH10L (solid ellipses), and B. subtilis 168 cssS::Sp htrB::pMutin4 containing pKTH10L (open ellipses). Samples for the determination of β-galactosidase activities (indicated in nanomoles per minute per OD600 unit) were withdrawn at the times indicated. Zero time (t = 0) indicates the transition point between the exponential and postexponential growth phases. The parental strain B. subtilis 168 was used as a negative control (data not shown).