FIG. 6.

Analysis of cssS transcription on CssS overexpression. A transcriptional cssS-lacZ gene fusion was used to determine the time courses of cssS expression in cells grown at 37°C in TY medium. The strains used for the analyses were B. subtilis 168 cssS::pMutin2 (BV2006; solid rectangles) and B. subtilis 168 cssS::pMutin2 amyE::XcssS (BV2023) in the absence (solid ellipses) or presence (open ellipses) of 1% xylose. The parental strain 168 was used as a negative control (data not shown). β-Galactosidase activities are indicated in nanomoles per minute per OD₆₀₀ unit. Zero time (t = 0) indicates the transition point between the exponential and postexponential growth phases.