TABLE 2.
Plasmids used in this study
| Plasmid | Characteristics | Reference |
|---|---|---|
| pUB110 | Kmr | 9 |
| pKTH10 | pUB110 derivative; encodes the α-amylase (AmyQ) of B. amyloliquefaciens; Kmr | 17 |
| pKTH10L | pKTH10 derivative; Kmr | 11 |
| pMutin4 | pBR322-based integration vector for B. subtilis; contains a multiple-cloning site downstream of the Pspac promoter and a promoterless lacZ gene preceded by the RBSa of the spoVG gene; Apr/Emr | 26 |
| pDL | Vector for the integration of transcriptional promoter-bgaB gene fusions in the amyE locus of B. subtilis; Apr Cmr | 30 |
| pDN220 | pDL containing the complete cssR-cssS control region on a PCR-amplified fragment; Apr Cmr | This study |
| pDN221 | pMutin4 containing the complete cssR-cssS control region on a PCR-amplified fragment; Apr Emr | This study |
| pDN222 | pMutin4 containing the complete cssR-cssS control region, with the A → G mutation at position −60 relative to the start codon of cssR; Apr Emr | This study |
| pDN223 | pDL containing the htrB control region with the T → C change at position −218 relative to the start codon of htrB; Apr Cmr | This study |
| pX | Vector for the integration of genes in the amyE locus; the integrated gene will be transcribed from the xylA promotor; carries the xylR gene; Apr Cmr | 12 |
| pXcssS | pX derivative; carries cssS downstream of the xylA promoter; Apr Cmr | This study |
a
RBS, ribosome-binding site