TABLE 3.
Expression of transcriptional fusions between the cssR-cssS promoter region and the bgaB reporter gene in various genetics background at 37 and 48°C
| Background strain | Presence of:
|
BgaB activitya at:
|
|||
|---|---|---|---|---|---|
| htrA | htrB | cssS | 37°C | 48°C | |
| DN220 | + | + | + | 1.2 | 1.8 |
| BV2017 | − | + | + | 3.6 | 6.9 |
| BV2019 | + | − | + | 2.6 | 3.9 |
| BV2021; suppressed | − | − | + | 3.7 | 8.2 |
| BV2016 | + | + | − | 0.8 | 1.0 |
| BV2018 | − | + | − | 0.6 | 0.7 |
| BV2020 | + | − | − | 1.6 | 1.5 |
| BV2022; suppressed | − | − | − | 0.8 | 1.1 |
a
All values represent BgaB activities that were determined 1 h after the cultures were split. One activity unit is defined as 1 nmol of o-nitrophenyl-β-d-galactopyranoside hydrolyzed per min per OD600 unit. All values represent the average of two independent experiments.