FIG. 3.

Secretion of YspG, YspH, and YspJ is blocked by mutations in yopN, yopP, and yopE, respectively. (A) Cultures were grown at 26°C in L medium supplemented with 290 mM NaCl to induce Ysp production. (B) Cultures were grown at 37°C in L medium depleted of calcium by the addition of 20 mM sodium oxalate and 20 mM MgCl₂ to induce Yop production. Lanes: 1, molecular mass markers; 2, JB580v (Wild Type); 3, GY4764 (yopN); 4, GY4618 (yopP); 5, GY4765 (yopE); 6, GY4428 (ysaV); 7, YVM356 (yscR); 8, GY4488 (pYVe8081⁻). The locations of selected Ysps and Yops are assigned according to size on the left of each panel. The secreted proteins were isolated and analyzed as described in the legend to Fig. 1. The molecular mass standards are approximately 77, 50, 35, and 20 kDa.