TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| DivIVA constructs | ||
| pWM1396 | Camr, pACYC184-based replicon with PBAD-divIVA::GFP | This study |
| pZD6 | Crbr Kanr, pMMB22 (IncQ) with Ptac-divIVA::GFP | This study |
| pZD7 | Crbr, pMMB22 with Ptac-divIVA::E2 | This study |
| pZD22 | Crbr, pMMB22 with Ptac-divIVA::B10 | This study |
| pZD1396 | Camr, pACYC184 replicon with PBAD-divIVA::LZ | This study |
| pZD1397 | Camr, pACYC184 replicon with PBAD-divIVA::E2 | This study |
| pZD1398 | Camr, pACYC184 replicon with PBAD-divIVA::E1 | This study |
| pZD1401 | Camr, Kanr, pWM1396 with a Kan cassette | This study |
| pPC1308 | Camr, pACYC184 replicon with PBAD-divIVA::B8 | This study |
| pPC1309 | Camr, pACYC184 replicon with PBAD-divIVA::B9 | This study |
| pPC1310 | Camr, pACYC184 replicon with PBAD-divIVA::B10 | This study |
| pKA11 | Camr, pACYC184 replicon with PBAD-divIVA | This study |
| pXZ9104 | Crbr, pBKS+.NdeI (ColE1 replicon) with PvirB-B10 | X.-R. Zhou |
| pXZ721-pXZ729 | Crbr, pBSK+.NdeI with Plac-virE2.i31 insertion alleles | 55 |
| pZD701-pZD709 | Camr, pACYC184 replicon with PBAD-divIVA fused in-frame to 3′ fragments of virE2 | This study |
| pZD791-pZD799 | Camr, pACYC184 replicon with PBAD-divIVA fused in-frame to 5′ fragments of virE2 | This study |
| pZD37 | Crbr, pBSK+ with Plac-virB10.63::phoA | This study |
| pZD39 | Camr, pACYC184 replicon with PBAD-divIVA::virB10.63::phoA | This study |
| pZD42 | Camr, pACYC184 replicon with PBAD-divIVA::virB10.63 | This study |
| FtsZ constructs | ||
| pWM976 | Camr, pACYC184 replicon with PBAD-ftsZ::GFP | 33 |
| pZD2 | Camr, pACYC184 replicon with PBAD-ftsZ::LZ | This study |
| pZZ7 | Camr, pACYC184 replicon with PBAD-ftsZ::E1 | This study |
| pZZ10 | Camr, pACYC184 replicon with PBAD-ftsZ::E2 | This study |
| GFP constructs | ||
| pXZ63 | Crbr, pBSK+.NdeI with Plac-GFP | 55 |
| pXZ66 | Kanr, pBSK+.NdeI with Plac-E2::GFP | 55 |
| pXZ168 | Crbr, pBSIIKS+.NdeI with Plac-E1::GFP | 55 |
| pZD1 | Crbr, pBSK+.NdeI with Plac-GFP::LZ | This study |
| pZD9 | Crbr, pBKS+.NdeI with PvirB-E1::GFP | This study |
| pZD12 | Kanr, pBKS+.NdeI with PvirB-E1::GFP | This study |
| pZD20 | Kanr, pBR322 replicon, with Ptrc-GFP::B10 | This study |
| pZD23 | Crbr, pBR322 replicon, with Ptrc-GFP::B10::phoA | This study |
| pZD40 | Crbr, pBSK+ with Ptrc-GFP::virB10.63::phoA | This study |
| pZD43 | Crbr, pBSK+ with Ptrc-GFP::virB10.63 | This study |
| pZD45 | Crbr, pBR322 with Ptrc-GFP::phoA | This study |
| pZD169 | Crbr, pBSIIKS+.NdeI with Plac-E1::GFP and Plac-E2 | This study |
| pZD170 | Crbr, pBSIIKS+.NdeI with Plac-E1::GFP and Plac-E1 | This study |
| pZZ9 | Crbr, pBKS+.NdeI with PvirB-E1 | 54 |
| pDSW207 | Crbr, pBR322 (ColE1 replicon) with Ptrc-GFP | 9 |
| pKA1 | Crbr, pBR322 with Ptrc-GFP::B10 | This study |
| pKA2 | Crbr, pBR322 with Ptrc-GFP::B8 | This study |
| pKA4 | Crbr, pBR322 with Ptrc-GFP::B9 | This study |
a
Drug resistance phenotype abbreviations: Cam, chloramphenicol; Crb, carbenicillin; Kan, kanamycin.