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Analysis of the apparent size of the RsbW-σB complex by gel filtration. The RsbW dimer and σB were mixed in an equimolar ratio and loaded onto a Superose-12 gel filtration column (Pharmacia). (A) Samples of the fractions were separated by SDS-12% PAGE and blotted with anti-RsbW and anti-σB polyclonal antibodies. (B) The intensities of the σB bands were measured by using Adobe Photoshop software after digital scanning of the autoradiographic film.
