FIG. 6.
Effect of the presence of ATP on the partition of RsbW between RsbV and σB. During the course of the phosphorylation of 15 μM RsbV by 1 μM RsbW dimer in the presence of 1 mM ATP and 3 mM MgCl2 and in the absence (left) or in the presence (right) of σB (15 μM), samples were taken at time intervals as indicated above the gel and subjected to nondenaturing 12% PAGE. Mixtures with an equimolar ratio of the RsbW monomer to RsbV and an equimolar ratio of the RsbW dimer to σB (first and last lanes, respectively) were used as markers of the positions of the two complexes.