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Tethered cells analysis of wild-type cells grown aerobically on sodium succinate medium to oxygen step changes. Each graph represents the average behavior of at least 20 cells. The arrows indicate addition (downward arrowhead) and removal (upward arrowhead) of oxygenated buffer. Either 2 mM sodium succinate (A) or 5 mM fructose (B) was used as steady background during buffer transition.
