FIG. 1.

Schematic representation of the ben-cat1 and cat2 genes. The open arrows indicate locations and directions of transcription of ORFs. IS1599 is indicated by a gray box. The orientation of the open arrow in IS1599 indicates the direction of transcription of the ORF within it. The thick solid line above the gene map indicates the DNA fragment from plasmids pBB311, pBP144, pCASL3, pPCA2, and pCAD that were sequenced in both strands. The open box indicates the omega cassette used for gene disruption. Abbreviations for restriction endonucleases: A, ApaI; B, BamHI; ET22, EcoT22I; EV, EcoRV; H, HindIII; N, NotI; Na, NaeI; Nc, NcoI; P, PstI; S, SalI, Sc, SacI; Sm, SmaI; Sp, SphI; Xb, XbaI; X, XhoI. aa, amino acids.