TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| Burkholderia sp. strain TH2 | BA+ 2CB+ 3CB− 4CB− | 44 |
| Burkholderia sp. strain NDBA1 | BA− 2CB− 3CB− 4CB− | 12 |
| Burkholderia sp. strain TD2 | cbd disruptant, BA− 2CB− 3CB− 4CB− | 44 |
| Burkholderia sp. strain TCD1 | cat1 disruptant of TH2 | This study |
| Burkholderia sp. strain TCD2 | cat2 disruptant of TH2 | This study |
| Burkholderia sp. strain TBD1 | benD disruptant of TH2 | This study |
| E. coli DH5α | supE44 ΔlacU169(φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 37 |
| E. coli S17-1 | recA thi pro hsdR−M+ pR4::2- Tc::Mu-Km::Tn7(Tpr/Smr) | 43 |
| E. coli BL21(DE3) | F−ompT hsdSB(rB− mB−) gal dcm (DE3) | Novagen |
| Plasmids | ||
| pBluescript II KS(+) | Apr, cloning vector | 37 |
| pET14b | Apr, Expression vector | Novagen |
| pET16b | Apr, Expression vector | Novagen |
| pJRD215 | Kmr Smr, broad-host-range vector with multiple-cloning sites | 6 |
| pMOB3 | Kmr Cmr, mob+sacB+ | 40 |
| pNOT322 | Apr, cloning vector | 40 |
| pHP45Ωaac | Gmr | 2 |
| pBP144 | 8-kbp PstI fragment, hybridized with the benA probe, cloned into PstI site of pBluescript II KS(+) | This study |
| pBB311 | 7.2-kbp BamHI fragment, hybridized with the benA probe, cloned into BamHI site of pBluescript II KS(+) | This study |
| pJPBC | 8-kbp KpnI-XbaI fragment of pBP144 cloned into KpnI-XbaI site of pJRD215 | This study |
| pPCA2 | 5.2-kbp PstI fragment, hybridized with the catA2 probe, cloned into PstI site of pBluescript II KS(+) | This study |
| pCAD | 3.7-kbp SalI fragment, hybridized with the SalI-PstI fragment probe from pPCA2, cloned into SalI site of pBluescript II KS(+) | This study |
| pCAU | 8-kbp NotI fragment, hybridized with the NotI-PstI fragment probe from pPCA2, cloned into NotI site of pBluescript II KS(+) | This study |
| pCASL3 | 2.3-kbp SalI-NotI fragment of pCAU cloned into the SalI site of pBluescript II KS(+) | This study |
| p14CA1 | 2.5-kbp XhoI fragment of pBB311 cloned into the XhoI site of pET14b | This study |
| p14CA2 | 3.3-kbp NcoI-XhoI fragment of pPCA2 cloned into the NcoI-XhoI site of pET14b | This study |
| pCA218 | 5.2-kbp PstI fragment of pPCA2 cloned into the PstI site of pUC18 | This study |
| pCA218Ω | pCA218 derivative; Apr Gmr | This study |
| pNCA218Ω | pNOT322 derivative containing the XbaI-SmaI insert of pCA218Ω; Apr Gmr | This study |
| pMNCA218Ω | pNCA218Ω derivatives; mob+sacB+ Apr Gmr | This study |
| pCB18 | 7.2-kbp BamHI fragment of pBB311 cloned into the BamHI site of pUC18 | This study |
| pCB18Ω | pCB18 derivative; Apr Gmr | This study |
| pNCB18Ω | pNOT322 derivative containing the XbaI-SmaI insert of pCB18Ω; Apr Gmr | This study |
| pMNCB18Ω | pNCB18Ω derivatives; mob+sacB+ Apr Gmr | This study |
| pDB90 | 9-kbp BamHI fragment, encoding benD, cloned into pBluescript II KS(+) | This study |
| pDBSC | pDB90 derivative: Apr | This study |
| pDBSCΩ | pDBSC derivative: Apr Gmr | This study |
| pNDBSCΩ | pDBSCΩ derivatives: Apr Gmr | This study |
| pMNDBSCΩ | pNDBSCΩ derivatives: mob+sacB+ Apr Gmr | This study |
| p16TLXYZ | XbaI-BamHI fragment encoding xylXYZ cloned into the XbaI-BamHI site of pET16b | This study |