TABLE 3.
Activity of catechol 1,2-dioxygenases with different substrates
| Substrate | Relative activitya (%)
|
Sp actb (nmol min−1 mg of crude protein−1)
|
||
|---|---|---|---|---|
| CatA1 | CatA2 | CatA1 | CatA2 | |
| Catechol | 100 | 100 | 94.8 | 3,920 |
| 3-Chlorocatechol | 4.4 | <0.1 | ||
| 4-Chlorocatechol | 18.1 | 2.5 | ||
| 4-Methylcatechol | 39.5 | 13.1 | ||
a
Expressed as a percentage of the specific activity with catechol which is set at 100%.
b
Catechol 1,2-dioxygenase activity was assayed spectrophotometrically at 260 nm with ring cleavage product inferred to be CCMs.