TABLE 2.
Acyl-HSL synthesis by B. cepacia complex isolates
| Isolate | Doubling timea (h) | Most abundant acyl-HSLb | Acyl-HSL radiolabele (cpm/30 min) | ceplf-(kb) | bvilf-(kb) |
|---|---|---|---|---|---|
| Genomovar I | |||||
| C9139 | 1.1 | C8-HSL | 6,370 | 2.2 | — |
| ATCC 25416 | 0.9 | C8-HSL | 8,480 | 2.2 | — |
| ATCC 17759 | 1.0 | C8-HSL | 17,329 | 2.2 | — |
| ATCC 10856 | 1.0 | C8-HSL | 7,193 | 2.2 | — |
| CEP509 | 1.0 | C8-HSL | 685 | 2.2 | — |
| LMG 17997 | 1.2 | ND | ND | 2.2 | — |
| Genomovar II | |||||
| C5274 | 1.1 | ND | ND | — | — |
| C5393 | 1.7 | — | 0 | — | — |
| 249-2 | 1.3 | — | 0 | — | — |
| ATCC 17616 | 1.1 | C8-HSL | 134 | — | — |
| C1576 | 1.8 | C8-HSL | 744 | — | — |
| JTC | 1.4 | — | 0 | — | — |
| LMG 13010 | 1.4 | — | 0 | — | — |
| C1962 | 1.1 | C8-HSL | 148 | — | — |
| CF-A1-1 | 1.5 | — | 0 | — | — |
| Genomovar III-A | |||||
| C1257 | 1.2 | C8-HSL | 12,894 | 6.7 | — |
| C4455 | 1.2 | F32c/C8-HSL | 343/253 | 8.0 | — |
| C5424 | 1.7 | — | 0 | >12.2 | — |
| C6433 | 1.2 | — | 0 | 6.7 | — |
| J2315 | 2.8 | — | 0 | >12.2 | — |
| BC7 | 3.0 | C8-HSL | 909 | >12.2 | — |
| K56-2 | 1.7 | C8-HSL | 301 | >12.2 | — |
| Genomovar III-B | |||||
| PC184 | 2.4 | C8-HSL | 345 | 6.7 | — |
| C1394 | 1.9 | C8-HSL | 1,964 | 7.1 | — |
| J415 | 2.4 | C8-HSL | 5,182 | 11.0 | — |
| CEP511 | 1.4 | — | ND | 6.7 | — |
| ATCC 17765 | 1.6 | C4-/3OC6-HSLd | 1,510 | 6.7 | — |
| Genomovar IV | |||||
| C7322 | 3.7 | ND | ND | 7.1 | — |
| ATCC 35254 | 2.8 | C8-HSL | 8,830 | 7.1 | — |
| LMG 07000 | 1.7 | C8-HSL | 8,272 | 7.7 | — |
| LMG 14294 | 1.8 | C8-HSL | 1,455 | 8.0 | — |
| LMG 14086 | 3.5 | C8-HSL | 160 | 7.7 | — |
| LMG 18888 | 2.1 | — | 0 | 7.7 | — |
| Genomovar V | |||||
| C2822 | 1.3 | C8-HSL | 17,931 | 5.5 | 2.8/6.7 |
| C9178 | 1.3 | C8-HSL | 26,519 | 5.5 | 2.8/6.7 |
| PC259 | 1.5 | C8-HSL | 2,592 | >12.2 | 2.8/6.7 |
| LMG 10929 | 1.3 | C10-HSL | 71,206 | >12.2 | 2.8/6.7 |
| FC441 | 1.3 | C8-HSL | 24,033 | >12.2 | 2.8/6.7 |
| LMG 16232 | 1.2 | C8-HSL | 175 | >12.2 | 2.8/6.7 |
| G4 | 2.0 | C10-HSL | 56,852 | >12.2 | 4.1/6.7 |
a
Doubling time in LB at 37°C, 250 rpm.
b
ND, not determined; —, no acyl-HSL detected.
c
Unidentified 14C-labeled species occurring in HPLC fraction 42.
d
C4-HSL and 3OC6-HSL eluted in the same fractions under the HPLC conditions used.
e
Amount of radiolabel detected in most abundant acyl-HSL in 5 ml of 14C-labeled culture.
f
Sizes of bands containing the specific gene sequence detected by Southern hybridization of gene probe to XhoI-digested chromosomal DNA; —, no hybridization.