TABLE 1.
Bacterial strains and plasmids used in this study
| Name | Derivation | Relevant properties | Source or reference |
|---|---|---|---|
| Parental and control strains | |||
| P90C | F−ara Δ(gpt-lac)5 thiA | Parental strain | 41 |
| FC29 | P90C/ F′ Δ(lacI lacZ) LacY+ Pro+ | Nonrevertible Lac− scavenger | 4; J. H. Miller, personal communication |
| FC36 | P90C Rifr | Spontaneous Rifr mutant of P90C | 4 |
| FC40 | FC36/F′ Φ(lacI33-lacZ) LacY+ Pro+ | Revertible Lac− strain | 4, 6 |
| FCJ64 | FC29 traD411::Km | traD nonrevertible Lac− scavenger | P. L. Foster, unpublished |
| FCJ65 | FC40 traD411::Km | traD revertible Lac− strain | 21 |
| CRS183 | FC40 recA938::Cm | recA revertible Lac− strain | This study |
| Strains with the gIIp nick site next to Φ(lacI33-lacZ) | |||
| CRS18, CRS241 | FC40 with gIIp nick site | FC40 + nick site | This study |
| CRS186, CRS237 | CRS18 recA938::Cm | recA FC40 + nick site | This study |
| CRS174 | P90C/F′ Φ(lacI33-lacZ) with nick site, traD411::Km | traD revertible Lac− strain + nick site | This study |
| CRS189 | CRS174 recA938::Cm | recA traD revertible Lac− strain + nick site | This study |
| CRS242 | CRS241 traD411::Km | traD FC40 + nick site | This study |
| CRS238 | CRS18 ΔruvC::Km | ruvC FC40 + nick site | This study |
| Strains carrying the empty vector, pCRS3 | |||
| CRS232 | CRS18/pCRS3 | FC40 + nick site/pPBAD vector | This study |
| CRS234 | F29/pCRS3 | Scavenger/pPBAD vector | This study |
| CRS243 | CRS241/pCRS3 | FC40 + nick site/pPBAD vector | This study |
| CRS245 | CRS242/pCRS3 | traD FC40 + nick site/pPBAD vector | This study |
| CRS249 | FCJ64/pCRS3 | traD scavenger/pPBAD vector | This study |
| CRS247 | FC40/pCRS3 | FC40/pPBAD vector | This study |
| CRS251 | FCJ65/pCRS3 | traD FC40/pPBAD vector | This study |
| Strains carrying the gene II plasmid, pCRS4 | |||
| CRS10 | CRS18/pCRS4 | FC40 + nick site/pPBAD-gene II | This study |
| CRS177 | CRS174/pCRS4 | traD revertible Lac− strain + nick site/pPBAD-gene II | This study |
| CRS179, CRS248 | FC40/pCRS4 | FC40/pPBAD-gene II | This study |
| CRS184 | CRS183/pCRS4 | recA FC40/pPBAD-gene II | This study |
| CRS187 | CRS186/pCRS4 | recA FC40 + nick site/pPBAD-gene II | This study |
| CRS190 | CRS189/pCRS4 | traD recA revertible Lac− strain + nick site/pPBAD-gene II | This study |
| CRS235 | F29/pCRS4 | Scavenger/pPBAD-gene II | This study |
| CRS244 | CRS241/pCRS4 | FC40 + nick site/pPBAD-gene II | This study |
| CRS246 | CRS242/pCRS4 | traD FC40 + nick site/pPBAD-gene II | This study |
| CRS250 | FCJ64/pCRS4 | traD scavenger/pPBAD-gene II | This study |
| CRS252 | FCJ65/pCRS4 | traD FC40/pPBAD-gene II | This study |
| Plasmids | |||
| pCRS3 | pBR322 carrying araC-PBAD regulatory region; Cbr Tcr | Control vector | This study |
| pCRS4 | pCRS3 carrying gene II; Cbr Tcr | Gene II under control of PBAD | This study |