TABLE 2.
Oligonucleotides used in this studya
| Oligo-nucleotide no. | Sequence |
|---|---|
| 1 | 5′CAGGAAACAGCTATGAC |
| 2 | 5′GTTTTCCCAGTCACGAC |
| 3 | 5′GATCCTCTTT*AATAGTGGACTCTTGTTCCAAACTGGAACAACCCG |
| 4 | 3′GAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGGGCCTAG |
| 5 | 5′ATCTCTCCAGGATCCTCGAATGGTGCAAAACC |
| 6 | 5′GCAGGAGCTCGTTATC |
a
Oligonucleotides 1 and 2 are, respectively, the universal M13 reverse primer (Invitrogen Corp.) and M13-40 forward primer (New England Biolabs). Oligonucleotides 3 and 4 contain the recognition site for nicking by the gIIp of bacteriophage f1; the site at which the DNA is nicked is indicated by an asterisk (11, 52). The terminal Sau3AI sites are underlined. Oligonucleotide 5 has a left tail of 8 bases, the restriction site for BamHI (underlined), and then 17 bases of the lacI gene (bases 8 to 24 of Ecolac [GenBank J01636.1]). Oligonucleotide 6 is 16 bases of lacZ (bases 3244 to 3228 of Ecolac).