TABLE 3.
Sequences of left and right endpoints after integration of pSET152 in S. coelicolor and S. lividans strainsb
| Clone | Rescued plasmid | Sequence of left endpoint | Sequence of right endpoint | Integration site(s) | Cosmid location(s)a |
|---|---|---|---|---|---|
| J1929(pSET152) | pSET152 | GGGCGTGCCCTTgagttctc | ggtaacctTTGGGCTCCCCG | attB | AC2 |
| PCS412 | pPC4121 | GGGGGTTACGTTgagttctc | ggtaacctTTTGCCTCTCCC | pseB1/pseB2 | E9 and H63 |
| pPC4122 | GGGAGAAGCTTTgagttctc | ggtaacctTTCGGCACGCCG | pseB2/pseB3 | H63 and D82 | |
| PCS418 | pPC4184 | GGGAGAAGCTTCgagttctc | ggtaacctTTTGCCTCTCCC | pseB2 | H63 |
| PCS4110 | pPC41104 | GGGAGAAGCTTCgagttctc | ggtaacctTTTGCCTCTCCC | pseB2 | H63 |
| PCS131 | pPC1311 | GGGAGAAGCTTTgagttctc | ggtaacctTCTGCCTCTCCC | pseB2 | H63 |
| pPC1312 | GGGAGAAGCTTCgagttctc | ggtaacctTTTGCCTCTCCC | pseB2 | H63 | |
| pPC1313 | GGGAGAAGCTTCgagttctc | ggtaacctTTTGCCTCTCCC | pseB2 | H63 | |
| pPC1314 | GGGAGAAGCTTCgngttctn | ggtaacctTTTGCCTCTCCC | pseB2 | H63 | |
| pPC1315 | GGGAGAAGCTTCgagttctc | ggtaacctTTTGCCTCTCCC | pseB2 | H63 | |
| pPC1316 | GGGAGAAGCTTTgagttctc | ggtaacctTCTGCCTCTCCC | pseB2 | H63 | |
| PCS139 | pPC1391 | GGGAGAAGCTTCgagttctc | ggtaacctTTTGCCTCTCCC | pseB2 | H63 |
| pPC1392 | CGGTGAGGCGTTgagttctc | ggtaacctTTCGGCACGCCG | pseB3 | D82 | |
| pPC1396 | CGGTGAGGCGTTgagttctc | ggtaacctTTCGGCACGCCG | pseB3 | D82 |
a
Cosmid map is located at http://www.sanger.ac.uk/Projects/S_coelicolor/clone_map.shtml.
b
Lowercase letters denote attP sequences.