TABLE 1.
Primer sequences used in this study
| Name | Nucleotide sequencea (5′-3′) | Restriction site |
|---|---|---|
| SH1 | GAAGAATTCGTTTGGCTCGTAATTTTAAAAAATATCAATTC | EcoRI |
| SH2 | GGAGGATCCGAAATCGCCTCCTACTTGCGCTTAGTTTTTTTC | BamHI |
| SH3 | GAAGAATTCGTATAGACAAACTAAGAGGGCTACGAGATAGCC | EcoRI |
| SH4 | AAGAAGCTTTCTTGTTCCTTCCATTGAAGCAACCACTGTTG | HindIII |
| Sp1 | CGCGGATCCGGATCTTCACCTAGATCC | BamHI |
| Sp2 | CCGGAATTCGTTACAAATTGTTTCACTAAATTAAAG | EcoRI |
| Pi2 | CCCAAGCTTGCTGTCATGAAGTTGTCGCGG | HindIII |
| Pi3 | CGCGGATCCGGTATGGCTGCATCTATGGGTGC | BamHI |
| Pi4 | CCCAAGCTTGGTTGACCTGTGCGGTCAGC | HindIII |
| P2.i3 | CGCGGATCCAATAGCCCCGGCGGTTCAACG | BamHI |
| P2.i4 | CCCAAGCTTGCCCTTGTGCACCACCAAGC | HindIII |
| PclpP1 | AAACTGCAGCCCTAGAAACAAAGTCGAAATC | PstI |
| PclpP2 | CGCGGATCCCGTAAGCGCGTTCTCCACG | BamHI |
| pP2.1 | AAACTGCAGCACATCAAAAGTTCCCGCACC | PstI |
| pP2.5 | CGCGGATCCGAGATCTTCCCCATCCCATTTG | BamHI |
| P8 | GCTGTCTTAGAACCGAGCGGAG | |
| P9 | CGTTTAGTGAATTGCGAAACAGC | |
| clpP2.1 | CGCTCGTATATAAAATAGCTAG | |
| clpP2.2 | CGGTTGCCCTGTTTTTTCTGC | |
| clpP2.3 | CCAGACGTGCAAACGCTATGC | |
| clpP2.6 | CTTGTCCAATCTTCTGAAGCTGC |
a
Restriction sites are underlined.