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Profile of labeled AHLs in Rm1021 and Rm1021 sinI. Five-milliliter cultures were incubated with 5 μCi of [14C]methionine. The labeled AHLs were extracted, separated by HPLC, and then quantitated by scintillation counting. Elution times of AHL standards are indicated in parentheses: C4-HL (13 min), oxo-C6-HL (18 min), C6-HL (34 min), and C8-HL (55 min).
