TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Sinorhizobium meliloti | ||
| Rm1021 | SU47 str-21 | 28 |
| Rm11511 | Rm1021 sinI::KM | This work |
| Rm11512 | Rm1021 sinR::GM | This work |
| Agrobacterium tumefaciens | ||
| NTL4(pZLR4) | NT1 derivative carrying a traG::lacZ reporter fusion | 31 |
| Escherichia coli | ||
| MT616 | MT607 (pRK600) | 20 |
| DH5α | See source | Life Technologies |
| Plasmids | ||
| pSinR | pPCR-Script Amp SK (+) carrying the sinR ORF | This work |
| pSinR::GM | pSinR carrying sinR::GM | This work |
| pSinRlong | pPCR-Script Amp SK (+) carrying sinR and surrounding region | This work |
| pSinRlong::GM | pSinRlong carrying sinR::GM | This work |
| pSinIlong | pPCR-Script Amp SK (+) carrying sinI and surrounding region | This work |
| pSinIlong::KM | pSinIlong carrying sinI::KM | This work |
| pJQ200SmSp | Shuttle vector carrying sacB | 32 |
| pSinIlong::KM/sacB | pJQ200SmSp carrying sinI::KM | This work |
| pSinRlong::GM/sacB | pJQ200SmSp carrying sinR::GM | This work |