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. 2002 Oct;184(20):5781–5788. doi: 10.1128/JB.184.20.5781-5788.2002

FIG. 2.

FIG. 2.

F-encoded proteins TraJ, TraM, and TraY are not detectable in a cpxA101* background. Immunoblot analysis with polyclonal antisera directed against TraJ, TraM, and TraY was carried out. Lanes 1 and 2, E. coli MC4100 with (+) or without (−) pOX38-Km; lanes 3 to 5, cpxA (TR8), cpxR (TR51), and cpxA101* (TR189) strains containing pOX38-Km, resepctively. The positions of TraJ, TraM, and TraY are indicated on the right, and relevant molecular weight markers are indicated (in kilodaltons) on the left. The loading control indicated at the bottom of the figure is obtained from a protein that nonspecifically cross-reacts with the antiserum.