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. 2002 Oct;184(20):5781–5788. doi: 10.1128/JB.184.20.5781-5788.2002

FIG. 3.

FIG. 3.

Cell envelope stress induced by NlpE overexpression reduces TraJ and TraM levels. Immunoblot analysis with polyclonal antisera directed against TraJ and TraM was carried out. Lanes 1 to 3, wild-type (MC4100), cpxA101* (TR189), and degP (JMR201) E. coli containing pOX38-Km, respectively; lanes 4 and 5, E. coli MC4100 containing pOX38-Km and the control vector, pBR322, or pLD404, expressing NlpE. The positions of TraJ and TraM are indicated on the right, and the relevant molecular weight marker is indicated (in kilodaltons) on the left.