recA and clpP lonA mutations do not rescue TraJ expression in the cpxA101* mutant. Immunoblot analysis with polyclonal antiserum directed against TraJ. Lanes 1 and 2, E. coli MC4100 without (−) or with (+) pOX38-Km; lanes 3 to 5, pOX38-Tc in cpxA101* (TR189), cpxA101* recA (TR981), and cpxA101* clpP lonA (TR984) strains. The position of TraJ is indicated on the right, and the position of the relevant molecular weight marker is shown (in kilodaltons) on the left. The loading control indicated at the bottom of the figure is obtained from a protein that nonspecifically cross-reacts with the antiserum.