TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Genotype or descriptiona	Source or reference
Bacterial strains
MC4100	F−araD139 Δ(argF-lac)U169 rpsL150(Strr) relA1 flb5301 deoC1 ptsF25 rbsR	2
XK1200	Nalr F−lacΔU124 Δ(nadA araG gal attL)	34
TR8	MC4100 cpxA::cam	42
TR20	MC4100 cpxA101	This work
TR51	MC4100 cpxR::spc	42
TR189	MC4100 cpxA101 zii::Tn10 λRS88[degP-lacZ]	This work
TR981	MC4100 cpxA101 zii::Tn10 λRS88[degP-lacZ] recA	This work
TR984	MC4100 cpxA101 zii::Tn10 λRS88[degP-lacZ] clpP lonA	This work
JMR201	MC4100 degP::Tn10	A. E. Rizzitello and T. J. Silhavy
Plasmids
pOX38-Km	Kmr F tra region, Rep FIA replicon	4
pOX38-Tc	Tcr F tra region, Rep FIA replicon	1
pRS27	Tcr 9-kb partial EcoRI F fragmentin pSC101	50
pBAD24	Apr PBAD cloning vector	16
pBADTraJ	traJ coding region fused to PBAD	This work
pBADTraM	traM coding region fused to PBAD	This work
pBADTraY	traY coding region fused to PBAD	This work
pBR322	General cloning vector	New England Biolabs
pLD404	Apr NlpE in pBR322	51
pMC874	KmrlacZ+ Δplac	3
pMCJ211	KmrtraJ ΩlacZ FinP+traJ-lacZ reporter plasmid	57
pGEX-KG	IPTG-inducible GST fusion expression	Amersham Pharmacia Biotech
pLJ5-13	AprT7Φ10-finP fusion in pUC19	19
pSLF20	finP F derivative	24

^aStr^r, streptomycin resistant; Nal^r, nalidixic acid resistant; Km^r, kanamycin resistant; Tc^r, tetracycline resistant; Ap^r, ampicillin resistant.