TABLE 1.
Genotypes and sources of gonococcal strains used in this study
| Strain | Genotype (reference) | Sourcea |
|---|---|---|
| FA19 | Wild type | P. F. Sparling |
| WV30 | mtrR171 | FA6140 DNA × FA19 |
| WV31 | mtrR171 mtrD::kan | mtrD::kan PCR × WV30 |
| WV32 | penA4 | FA6140 DNA × FA19 |
| WV33 | penA4 mtrD::kan | mtrD::kan PCR × WV32 |
| WV34 | mtrR171 penA4 | FA6140 DNA × WV32 |
| WV35 | mtrR171 mtrD::kan penA4 | mtrD::kan PCR × WV34 |
| WV36 | mtrR171 penA4 pen B | FA6140 DNA × WV34 |
| WV37 | mtrR171 mtrD::kan penA4 penB | mtrD::kan PCR × WV36 |
| FA19AB | penA penB | R. Nicholas |
| WV38 | mtrR171 penA penB | FA6140 mtrR PCR × FA19AB |
| FA140 | mtrR140 penA2 penB2 (24) | P. F. Sparling |
| WV24 | mtrR140 mtrD::kan penA2 penB2 | mtrD::kan PCR × FA140 |
| FA6140 | mtrR171 penA4 penB ponA1 | P. F. Sparling |
| WV22 | mtrR171 mtrD:: kan penA4 penB ponA1 | mtrD:: kan PCR × FA6140 |
a
Transformants are shown as donor DNA × recipient strain, produced as described in Materials and Methods. PCR products were gel purified prior to transformation. Gene descriptions: mtrR encodes a transcriptional repressor of MtrCDE expression; mtrD encodes the transporter component of the MtrCDE efflux system; penA is the structural gene for PBP-2; penB is an allele of porB, encoding the gonococcal porin; and ponA is the structural gene for PBP-1.